Determination of the total energy budget of Hetaerina titia: application of a new analytical method to the study of seasonal and regional polyphenism

Presentation Type

Poster

Presentation Type

Submission

Keywords

Damselfly, polyphenism, melanization, assay, analytical chemistry, quantitative, ecology, energy, proteins, lipids, and carbohydrates

Department

Chemistry

Major

Chemistry

Abstract

Determination of the total energy budget of Hetaerina titia: application of a new analytical method to the study of seasonal and regional polyphenism

Coates, Kathleen M.; Marsh, Conner.; Graf, C. Foster; Grether, Gregory; Green, David B.

Observations that the Smoky Rubyspot damselfly, Hetaerina titia, exhibits seasonal and regional polyphenism and wing pigmentation, which has implications in behavior and reproductive success, has prompted interest into understanding the biochemical causation and energy budget consequences of varied pigmentation. We have developed analytical methods in a sequential series of semimicro spectroscopic analytical procedures targeting proteins, carbohydrates, and pooled fats, lipids and fatty acids to probe the total energy budget of these odinates as part of a broader study investigating differences in energy availability in H. titia males as a result of pigmentation, geographic, and seasonal differences. The determinations can be rapidly performed and we have validated each step in the analysis, demonstrating 95-107% recovery for each component class. We provide an overview of our method along with initial results pertaining to seasonal variations and geographic factors.

Faculty Mentor

David Green

Funding Source or Research Program

John Stauffer Charitable Trust

Location

Waves Cafeteria

Start Date

22-3-2024 1:30 PM

End Date

22-3-2024 2:30 PM

This document is currently not available here.

Share

COinS
 
Mar 22nd, 1:30 PM Mar 22nd, 2:30 PM

Determination of the total energy budget of Hetaerina titia: application of a new analytical method to the study of seasonal and regional polyphenism

Waves Cafeteria

Determination of the total energy budget of Hetaerina titia: application of a new analytical method to the study of seasonal and regional polyphenism

Coates, Kathleen M.; Marsh, Conner.; Graf, C. Foster; Grether, Gregory; Green, David B.

Observations that the Smoky Rubyspot damselfly, Hetaerina titia, exhibits seasonal and regional polyphenism and wing pigmentation, which has implications in behavior and reproductive success, has prompted interest into understanding the biochemical causation and energy budget consequences of varied pigmentation. We have developed analytical methods in a sequential series of semimicro spectroscopic analytical procedures targeting proteins, carbohydrates, and pooled fats, lipids and fatty acids to probe the total energy budget of these odinates as part of a broader study investigating differences in energy availability in H. titia males as a result of pigmentation, geographic, and seasonal differences. The determinations can be rapidly performed and we have validated each step in the analysis, demonstrating 95-107% recovery for each component class. We provide an overview of our method along with initial results pertaining to seasonal variations and geographic factors.