Presentation Type

Poster

Presentation Type

Submission

Keywords

folic acid, neurodegenerative cognitive model, locus coerulus

Department

Nutritional Science

Major

Nutritional Science

Abstract

DEFICIENT DIETARY FOLIC ACID AND SYSTEMIC DSP-4 INDUCED LOCUS COERULEUS LOSS ASSESSED AS NEURODEGENERATIVE COGNITIVE MODEL IN ADULT CB57/J6 MICE Gabriel Kong, C. Foster Graf, Aristotle Zeng, Theodore Helm, Simon Khorani, Rohan Rample, Smayana Kurapati, Riva Rainier, Aidan Schmidt, Maximilian Doane, David B. Green, and Susan E. Helm Chemistry and Nutritional Science, Pepperdine University, Malibu, California

Dietary deficiency of folic acid (FA) shortened nerve lengths as much as 50% in hippocampal dentate gyrus (DG). Locus coeruleus (LC) is largest noradrenergic nucleus in central nervous system with neurogenic projections widely dispersed in the brain and, notably, a major norepinephrine source in the DG and frontal cortex (FC). For 16 weeks, adult CB57/J6 mice (N=66) were provided 4 dietary/DSP-4 treatments: NFCS (n=16, normal FA control, saline injected [0.2 mg FA/kg BW]); NFCD (n=18, normal FA, DSP-4 injected (50 mg DSP-4/kg BW); FADS (n=16, FA deficient, saline injected); and, FADD (n=16, FA deficient, DSP-4 injected). NE in FC was measured in addition to significant loss of LC neurons; astrocytes, calcium signaling, and embryonic neurons using, respectively, immunohistochemical methods for tyrosine hydroxylase (TH), GABA fibrillary acidic protein (GFAP), parvalbumin (PV), doublecortin (DCX) were analyzed. Confirmation of phenotypic cognitive behavior with Nesting Behavior; decreased distance traveled with Novel Object Recognition (NOR); and, decreased rearing and latency to explore to the center of the field in Open Field Testing (OFT) were enumerated between NFCS and FADD (p

Faculty Mentor

Susan E. Helm

Funding Source or Research Program

Academic Year Undergraduate Research Initiative, Summer Undergraduate Research Program, Undergraduate Research Fellowship, Not Identified

Location

Waves Cafeteria

Start Date

22-3-2024 1:30 PM

End Date

22-3-2024 2:30 PM

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Mar 22nd, 1:30 PM Mar 22nd, 2:30 PM

DEFICIENT DIETARY FOLIC ACID AND SYSTEMIC DSP-4 INDUCED LOCUS COERULEUS LOSS ASSESSED AS NEURODEGENERATIVE COGNITIVE MODEL IN ADULT CB57/J6 MICE

Waves Cafeteria

DEFICIENT DIETARY FOLIC ACID AND SYSTEMIC DSP-4 INDUCED LOCUS COERULEUS LOSS ASSESSED AS NEURODEGENERATIVE COGNITIVE MODEL IN ADULT CB57/J6 MICE Gabriel Kong, C. Foster Graf, Aristotle Zeng, Theodore Helm, Simon Khorani, Rohan Rample, Smayana Kurapati, Riva Rainier, Aidan Schmidt, Maximilian Doane, David B. Green, and Susan E. Helm Chemistry and Nutritional Science, Pepperdine University, Malibu, California

Dietary deficiency of folic acid (FA) shortened nerve lengths as much as 50% in hippocampal dentate gyrus (DG). Locus coeruleus (LC) is largest noradrenergic nucleus in central nervous system with neurogenic projections widely dispersed in the brain and, notably, a major norepinephrine source in the DG and frontal cortex (FC). For 16 weeks, adult CB57/J6 mice (N=66) were provided 4 dietary/DSP-4 treatments: NFCS (n=16, normal FA control, saline injected [0.2 mg FA/kg BW]); NFCD (n=18, normal FA, DSP-4 injected (50 mg DSP-4/kg BW); FADS (n=16, FA deficient, saline injected); and, FADD (n=16, FA deficient, DSP-4 injected). NE in FC was measured in addition to significant loss of LC neurons; astrocytes, calcium signaling, and embryonic neurons using, respectively, immunohistochemical methods for tyrosine hydroxylase (TH), GABA fibrillary acidic protein (GFAP), parvalbumin (PV), doublecortin (DCX) were analyzed. Confirmation of phenotypic cognitive behavior with Nesting Behavior; decreased distance traveled with Novel Object Recognition (NOR); and, decreased rearing and latency to explore to the center of the field in Open Field Testing (OFT) were enumerated between NFCS and FADD (p

 

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