Presentation Type
Poster
Presentation Type
Submission
Keywords
folic acid, neurodegenerative cognitive model, locus coerulus
Department
Nutritional Science
Major
Nutritional Science
Abstract
DEFICIENT DIETARY FOLIC ACID AND SYSTEMIC DSP-4 INDUCED LOCUS COERULEUS LOSS ASSESSED AS NEURODEGENERATIVE COGNITIVE MODEL IN ADULT CB57/J6 MICE Gabriel Kong, C. Foster Graf, Aristotle Zeng, Theodore Helm, Simon Khorani, Rohan Rample, Smayana Kurapati, Riva Rainier, Aidan Schmidt, Maximilian Doane, David B. Green, and Susan E. Helm Chemistry and Nutritional Science, Pepperdine University, Malibu, California
Dietary deficiency of folic acid (FA) shortened nerve lengths as much as 50% in hippocampal dentate gyrus (DG). Locus coeruleus (LC) is largest noradrenergic nucleus in central nervous system with neurogenic projections widely dispersed in the brain and, notably, a major norepinephrine source in the DG and frontal cortex (FC). For 16 weeks, adult CB57/J6 mice (N=66) were provided 4 dietary/DSP-4 treatments: NFCS (n=16, normal FA control, saline injected [0.2 mg FA/kg BW]); NFCD (n=18, normal FA, DSP-4 injected (50 mg DSP-4/kg BW); FADS (n=16, FA deficient, saline injected); and, FADD (n=16, FA deficient, DSP-4 injected). NE in FC was measured in addition to significant loss of LC neurons; astrocytes, calcium signaling, and embryonic neurons using, respectively, immunohistochemical methods for tyrosine hydroxylase (TH), GABA fibrillary acidic protein (GFAP), parvalbumin (PV), doublecortin (DCX) were analyzed. Confirmation of phenotypic cognitive behavior with Nesting Behavior; decreased distance traveled with Novel Object Recognition (NOR); and, decreased rearing and latency to explore to the center of the field in Open Field Testing (OFT) were enumerated between NFCS and FADD (p
Faculty Mentor
Susan E. Helm
Funding Source or Research Program
Academic Year Undergraduate Research Initiative, Summer Undergraduate Research Program, Undergraduate Research Fellowship, Not Identified
Location
Waves Cafeteria
Start Date
22-3-2024 1:30 PM
End Date
22-3-2024 2:30 PM
Included in
DEFICIENT DIETARY FOLIC ACID AND SYSTEMIC DSP-4 INDUCED LOCUS COERULEUS LOSS ASSESSED AS NEURODEGENERATIVE COGNITIVE MODEL IN ADULT CB57/J6 MICE
Waves Cafeteria
DEFICIENT DIETARY FOLIC ACID AND SYSTEMIC DSP-4 INDUCED LOCUS COERULEUS LOSS ASSESSED AS NEURODEGENERATIVE COGNITIVE MODEL IN ADULT CB57/J6 MICE Gabriel Kong, C. Foster Graf, Aristotle Zeng, Theodore Helm, Simon Khorani, Rohan Rample, Smayana Kurapati, Riva Rainier, Aidan Schmidt, Maximilian Doane, David B. Green, and Susan E. Helm Chemistry and Nutritional Science, Pepperdine University, Malibu, California
Dietary deficiency of folic acid (FA) shortened nerve lengths as much as 50% in hippocampal dentate gyrus (DG). Locus coeruleus (LC) is largest noradrenergic nucleus in central nervous system with neurogenic projections widely dispersed in the brain and, notably, a major norepinephrine source in the DG and frontal cortex (FC). For 16 weeks, adult CB57/J6 mice (N=66) were provided 4 dietary/DSP-4 treatments: NFCS (n=16, normal FA control, saline injected [0.2 mg FA/kg BW]); NFCD (n=18, normal FA, DSP-4 injected (50 mg DSP-4/kg BW); FADS (n=16, FA deficient, saline injected); and, FADD (n=16, FA deficient, DSP-4 injected). NE in FC was measured in addition to significant loss of LC neurons; astrocytes, calcium signaling, and embryonic neurons using, respectively, immunohistochemical methods for tyrosine hydroxylase (TH), GABA fibrillary acidic protein (GFAP), parvalbumin (PV), doublecortin (DCX) were analyzed. Confirmation of phenotypic cognitive behavior with Nesting Behavior; decreased distance traveled with Novel Object Recognition (NOR); and, decreased rearing and latency to explore to the center of the field in Open Field Testing (OFT) were enumerated between NFCS and FADD (p