The effects of nitrate supplementation on oral microbiota composition in females

Presentation Type

Poster

Presentation Type

Submission

Keywords

beetroot, dietary nitrate, nitric oxide, microbiome

Department

Sports Medicine

Major

Sports Medicine

Abstract

Dietary nitrate (NO3-) supplementation, improves cardiovascular, cognitive, and contractile function by augmenting nitric oxide (NO) biosynthesis; however, the specific taxa responsible, and whether NO3- ingestion can promote a favorable microbial composition to facilitate NO synthesis has yet to be established. To determine whether NO3- supplementation is associated with specific microbial taxa in females, buccal cell samples were collected from participants across two experiments: 1) 14 healthy adult females consumed an acute dose of NO3--depleted beetroot juice (PL) or NO3--rich beetroot juice (BR); and 2) 13 sedentary Hispanic females consumed an acute dose of PL or BR combined with ascorbic acid (VITC) or a crystal light beverage (CRY). Buccal samples were isolated for their 16s rRNA gene. DNA concentration was assessed with the Nanodrop guidelines of > 10 ng/uL and A260/A280 values of 1.6 to 2.4. DNA concentration in experiment 1 (PL: 37.66±18.92; BR: 37.52±11.54 ng/uL) and experiment 2 (BR+VITC: 37.66±18.92; BR+CRY: 37.52±11.54 ng/uL; PL+VITC: 37.52±11.54; PL+CRY: 37.52±11.54) were adequate. DNA purity in experiment 1 (PL: 2.03±0.28; BR: 2.06±0.21) and experiment 2 (BR+VITC: 37.66±18.92; BR+CRY: 37.52±11.54 ng/uL; PL+VITC: 37.52±11.54; PL+CRY: 37.52±11.54) were adequate. Samples were amplified and checked using polymerase chain reaction and southern blots, respectively. Pooled samples are undergoing library sequencing to determine microbial taxa composition following NO3- supplementation.

Faculty Mentor

Dr. Rachel Tan

Funding Source or Research Program

Academic Year Undergraduate Research Initiative, Summer Undergraduate Research in Biology

Location

Waves Cafeteria

Start Date

24-3-2023 2:00 PM

End Date

24-3-2023 4:00 PM

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Mar 24th, 2:00 PM Mar 24th, 4:00 PM

The effects of nitrate supplementation on oral microbiota composition in females

Waves Cafeteria

Dietary nitrate (NO3-) supplementation, improves cardiovascular, cognitive, and contractile function by augmenting nitric oxide (NO) biosynthesis; however, the specific taxa responsible, and whether NO3- ingestion can promote a favorable microbial composition to facilitate NO synthesis has yet to be established. To determine whether NO3- supplementation is associated with specific microbial taxa in females, buccal cell samples were collected from participants across two experiments: 1) 14 healthy adult females consumed an acute dose of NO3--depleted beetroot juice (PL) or NO3--rich beetroot juice (BR); and 2) 13 sedentary Hispanic females consumed an acute dose of PL or BR combined with ascorbic acid (VITC) or a crystal light beverage (CRY). Buccal samples were isolated for their 16s rRNA gene. DNA concentration was assessed with the Nanodrop guidelines of > 10 ng/uL and A260/A280 values of 1.6 to 2.4. DNA concentration in experiment 1 (PL: 37.66±18.92; BR: 37.52±11.54 ng/uL) and experiment 2 (BR+VITC: 37.66±18.92; BR+CRY: 37.52±11.54 ng/uL; PL+VITC: 37.52±11.54; PL+CRY: 37.52±11.54) were adequate. DNA purity in experiment 1 (PL: 2.03±0.28; BR: 2.06±0.21) and experiment 2 (BR+VITC: 37.66±18.92; BR+CRY: 37.52±11.54 ng/uL; PL+VITC: 37.52±11.54; PL+CRY: 37.52±11.54) were adequate. Samples were amplified and checked using polymerase chain reaction and southern blots, respectively. Pooled samples are undergoing library sequencing to determine microbial taxa composition following NO3- supplementation.