The effects of nitrate supplementation on oral microbiota composition in females
Presentation Type
Poster
Presentation Type
Submission
Keywords
beetroot, dietary nitrate, nitric oxide, microbiome
Department
Sports Medicine
Major
Sports Medicine
Abstract
Dietary nitrate (NO3-) supplementation, improves cardiovascular, cognitive, and contractile function by augmenting nitric oxide (NO) biosynthesis; however, the specific taxa responsible, and whether NO3- ingestion can promote a favorable microbial composition to facilitate NO synthesis has yet to be established. To determine whether NO3- supplementation is associated with specific microbial taxa in females, buccal cell samples were collected from participants across two experiments: 1) 14 healthy adult females consumed an acute dose of NO3--depleted beetroot juice (PL) or NO3--rich beetroot juice (BR); and 2) 13 sedentary Hispanic females consumed an acute dose of PL or BR combined with ascorbic acid (VITC) or a crystal light beverage (CRY). Buccal samples were isolated for their 16s rRNA gene. DNA concentration was assessed with the Nanodrop guidelines of > 10 ng/uL and A260/A280 values of 1.6 to 2.4. DNA concentration in experiment 1 (PL: 37.66±18.92; BR: 37.52±11.54 ng/uL) and experiment 2 (BR+VITC: 37.66±18.92; BR+CRY: 37.52±11.54 ng/uL; PL+VITC: 37.52±11.54; PL+CRY: 37.52±11.54) were adequate. DNA purity in experiment 1 (PL: 2.03±0.28; BR: 2.06±0.21) and experiment 2 (BR+VITC: 37.66±18.92; BR+CRY: 37.52±11.54 ng/uL; PL+VITC: 37.52±11.54; PL+CRY: 37.52±11.54) were adequate. Samples were amplified and checked using polymerase chain reaction and southern blots, respectively. Pooled samples are undergoing library sequencing to determine microbial taxa composition following NO3- supplementation.
Faculty Mentor
Dr. Rachel Tan
Funding Source or Research Program
Academic Year Undergraduate Research Initiative, Summer Undergraduate Research in Biology
Location
Waves Cafeteria
Start Date
24-3-2023 2:00 PM
End Date
24-3-2023 4:00 PM
The effects of nitrate supplementation on oral microbiota composition in females
Waves Cafeteria
Dietary nitrate (NO3-) supplementation, improves cardiovascular, cognitive, and contractile function by augmenting nitric oxide (NO) biosynthesis; however, the specific taxa responsible, and whether NO3- ingestion can promote a favorable microbial composition to facilitate NO synthesis has yet to be established. To determine whether NO3- supplementation is associated with specific microbial taxa in females, buccal cell samples were collected from participants across two experiments: 1) 14 healthy adult females consumed an acute dose of NO3--depleted beetroot juice (PL) or NO3--rich beetroot juice (BR); and 2) 13 sedentary Hispanic females consumed an acute dose of PL or BR combined with ascorbic acid (VITC) or a crystal light beverage (CRY). Buccal samples were isolated for their 16s rRNA gene. DNA concentration was assessed with the Nanodrop guidelines of > 10 ng/uL and A260/A280 values of 1.6 to 2.4. DNA concentration in experiment 1 (PL: 37.66±18.92; BR: 37.52±11.54 ng/uL) and experiment 2 (BR+VITC: 37.66±18.92; BR+CRY: 37.52±11.54 ng/uL; PL+VITC: 37.52±11.54; PL+CRY: 37.52±11.54) were adequate. DNA purity in experiment 1 (PL: 2.03±0.28; BR: 2.06±0.21) and experiment 2 (BR+VITC: 37.66±18.92; BR+CRY: 37.52±11.54 ng/uL; PL+VITC: 37.52±11.54; PL+CRY: 37.52±11.54) were adequate. Samples were amplified and checked using polymerase chain reaction and southern blots, respectively. Pooled samples are undergoing library sequencing to determine microbial taxa composition following NO3- supplementation.