Botryosphaeria dothidea is an opportunistic fungus found in some of the cavitated branches of the vegetation in the Santa Monica Mountains. In this study, we focused on finding the presence of Botryosphaeria dothidea in Ceanothus spinosus located on Pepperdine University’s campus. We wanted to test this hypothesis because we observed a large amount of dieback in the Ceanothus spinosus population and wanted to identify a possible contributor. Because of the large number of cavitated branches the Ceanothus spinosus contained, we hypothesized they contained fungus. To test this, we took twenty samples of twenty different Ceanothus spinosus, isolated the fungus from the xylem of each sample and incubated the samples in petri dishes for the span of one week. After fungal growth was complete, we found that only 30% of our samples were showing evidence of the presence of fungus. To discover whether or not the fungus present was Botryosphaeria dothidea, we extracted a small amount of DNA from five of the samples showing fungal growth. Of those five samples, we chose the three with the highest concentration of DNA in the sample and sequenced them in order to find out if the fungus infecting the Ceanothus spinosus was Botryosphaeria dothidea.
Grinovich, Brandon R.; Puncochar, Chelsea N.; Carter, Katarina L.; Davis, Stephen D.; Honeycutt, Rodney L.; Cao, Talia A.; and Smith, Karagan L., "Testing for the Presence of Botryosphaeria dothidea in Ceanothus spinosus" (2017). Pepperdine University, Featured Research. Paper 216.