Presentation Title

Effect of Presence or Absence of Dietary Folic Acid upon mouse model of Alzheimer Disease, Pilot data

Presentation Type

Oral Presentation

Department

Nutritional Science

Abstract

Alzheimer’s disease impacts 18 million patients worldwide. An early indicator of Alzheimer's disease is the degeneration of locus ceruleus neurons and reduction of norepinephrine concentration in locus ceruleus projection areas. The neurotoxin N-(2- chloroethyl)-N- ethyl-bromo- benzylamine (DSP 4 ) has been demonstrated to cause severe damage to locus ceruleus neurons and cortical norepinephrine transporters in mice. It has been observed that mice injected with DSP4 also present with elevated amyloid amyloid b levels and impaired spatial memory performance when compared with control mice. To effectively examine Alzheimer's disease, It is critical to develop a model that mimics the amyloid b (Ab) and tau pathology as well as the neuroinflammatory component of the disease. Using our pilot data, we designed an experiment (ongoing) to measure the effects of chronic folic acid deficiency in a mouse model of Azheimer's disease. To accomplish this, we have injected the mice with either saline (control), or 10 mg/kg DSP 4 (low dose) or, 50 mg/kg DSP 4 (high dose); then testing the C57bl mice using several behavioral and cognitive tests (ability to build a nest with Nestlets; occurrence of rearing using Open Field testing [OF]; curiosity using Novel Object Recognition[NOR]) during the spring and summer of 2018. In summer of 2017, we performed pilot tests by injecting C57bl/6 mice with varying levels of DSP 4 in order to determine the optimal dosage that manifests accurate rearing in our OF testing, a recognized measurement of Alzheimer’s disease in the mouse model. The DSP 4 injected mice used for the pilot tests were placed in OF tests, an experiment commonly used to measure general locomotor activity levels and anxiety in rodents. Data for the pilot study demonstrated that a significant portion of the mice died if injected with 50mg/kg DSP 4 within 3 day intervals. Mice with one injection of 50mg/kg DSP 4 did not show significance in overall 15 minute OF tests with regards to rearing or line crossings when compared with mice that were injected with saline. Indeed, significance occurred when data points were compared using minute by minute’s intervals.

Faculty Mentor

Dr. Susan Helm

Presentation Session

Session C

Location

BPC 190

Start Date

23-3-2018 4:45 PM

End Date

23-3-2018 5:00 PM

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Mar 23rd, 4:45 PM Mar 23rd, 5:00 PM

Effect of Presence or Absence of Dietary Folic Acid upon mouse model of Alzheimer Disease, Pilot data

BPC 190

Alzheimer’s disease impacts 18 million patients worldwide. An early indicator of Alzheimer's disease is the degeneration of locus ceruleus neurons and reduction of norepinephrine concentration in locus ceruleus projection areas. The neurotoxin N-(2- chloroethyl)-N- ethyl-bromo- benzylamine (DSP 4 ) has been demonstrated to cause severe damage to locus ceruleus neurons and cortical norepinephrine transporters in mice. It has been observed that mice injected with DSP4 also present with elevated amyloid amyloid b levels and impaired spatial memory performance when compared with control mice. To effectively examine Alzheimer's disease, It is critical to develop a model that mimics the amyloid b (Ab) and tau pathology as well as the neuroinflammatory component of the disease. Using our pilot data, we designed an experiment (ongoing) to measure the effects of chronic folic acid deficiency in a mouse model of Azheimer's disease. To accomplish this, we have injected the mice with either saline (control), or 10 mg/kg DSP 4 (low dose) or, 50 mg/kg DSP 4 (high dose); then testing the C57bl mice using several behavioral and cognitive tests (ability to build a nest with Nestlets; occurrence of rearing using Open Field testing [OF]; curiosity using Novel Object Recognition[NOR]) during the spring and summer of 2018. In summer of 2017, we performed pilot tests by injecting C57bl/6 mice with varying levels of DSP 4 in order to determine the optimal dosage that manifests accurate rearing in our OF testing, a recognized measurement of Alzheimer’s disease in the mouse model. The DSP 4 injected mice used for the pilot tests were placed in OF tests, an experiment commonly used to measure general locomotor activity levels and anxiety in rodents. Data for the pilot study demonstrated that a significant portion of the mice died if injected with 50mg/kg DSP 4 within 3 day intervals. Mice with one injection of 50mg/kg DSP 4 did not show significance in overall 15 minute OF tests with regards to rearing or line crossings when compared with mice that were injected with saline. Indeed, significance occurred when data points were compared using minute by minute’s intervals.