Presentation Title

Analytical- and Preparative-Scale Isolation of Oleandrin from Nerium Oleander using Microwave-Assisted Extraction Coupled with Purification using Supported-Metal Ion Solid Phase Extraction Resins

Presentation Type

Poster

Keywords

oleandrin, analytical chemistry, isolation, purification, chemistry, oleander, HPLC, NMR, solid phase extraction, resin

Department

Chemistry

Major

Chemistry with emphasis in Biochemistry

Abstract

We have developed a new class of supported-metal ion solid-phase extraction resins to target the extraction of glycosidic compounds from plant leaf tissue. We show that these supported-ion SPE resins can selectively isolate oleandrin, from Nerium oleander, in high yield and purity utilizing fewer and less hazardous solvents over published liquid-liquid extraction methods. In addition, using microwave-assisted extraction (MAE) significantly decreases the total time required to isolate the oleandrin and reduces the volume of solvents necessary by more than a factor of two. We demonstrate the practicality of scaling the isolation from the analytical scale to milligram quantities of oleandrin. The purity of the isolated oleandrin is verified with NMR spectroscopy by comparison to standard oleandrin.

Faculty Mentor

Dr. David B. Green

Funding Source or Research Program

Not Identified

Location

Waves Cafeteria

Start Date

24-3-2017 2:00 PM

End Date

24-3-2017 3:00 PM

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Mar 24th, 2:00 PM Mar 24th, 3:00 PM

Analytical- and Preparative-Scale Isolation of Oleandrin from Nerium Oleander using Microwave-Assisted Extraction Coupled with Purification using Supported-Metal Ion Solid Phase Extraction Resins

Waves Cafeteria

We have developed a new class of supported-metal ion solid-phase extraction resins to target the extraction of glycosidic compounds from plant leaf tissue. We show that these supported-ion SPE resins can selectively isolate oleandrin, from Nerium oleander, in high yield and purity utilizing fewer and less hazardous solvents over published liquid-liquid extraction methods. In addition, using microwave-assisted extraction (MAE) significantly decreases the total time required to isolate the oleandrin and reduces the volume of solvents necessary by more than a factor of two. We demonstrate the practicality of scaling the isolation from the analytical scale to milligram quantities of oleandrin. The purity of the isolated oleandrin is verified with NMR spectroscopy by comparison to standard oleandrin.