Presentation Title

Solid-phase extraction using new divalent metal ion-loaded cation exchange resins and application to the isolation of oleandrin from Nerium oleander

Presentation Type

Poster

Department

Chemistry

Major

Chemistry

Abstract

Oleandrin, which is found in the plant Nerium oleander, is known to induce fatal poisoning by ingestion of small quantities; however, this cardiac glycoside is also known for its medical and toxicological properties that make it useful in treating heart failure and cardiac arrhythmia. We have developed a new class of supported metal ion solid-phase extraction resins to isolate oleandrin in high purity from N. oleander, utilizing fewer and less hazardous solvents and accompanied with a factor of 3 time savings over published liquid-liquid extraction methods from plant tissue. We demonstrate quantitative recovery and observed improved selectivity with calcium- and copper(II)-modified resins, as compared to commercially-available unmodified resins. We also present results showing a further improvement in extraction time using microwave-assisted extraction. Work is on-going to expand the applicability of this new class of resins to other glycosides.

Faculty Mentor

Dr. David Green

Funding Source or Research Program

Academic Year Undergraduate Research Initiative, Summer Undergraduate Research Program, Undergraduate Research Fellowship

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Solid-phase extraction using new divalent metal ion-loaded cation exchange resins and application to the isolation of oleandrin from Nerium oleander

Oleandrin, which is found in the plant Nerium oleander, is known to induce fatal poisoning by ingestion of small quantities; however, this cardiac glycoside is also known for its medical and toxicological properties that make it useful in treating heart failure and cardiac arrhythmia. We have developed a new class of supported metal ion solid-phase extraction resins to isolate oleandrin in high purity from N. oleander, utilizing fewer and less hazardous solvents and accompanied with a factor of 3 time savings over published liquid-liquid extraction methods from plant tissue. We demonstrate quantitative recovery and observed improved selectivity with calcium- and copper(II)-modified resins, as compared to commercially-available unmodified resins. We also present results showing a further improvement in extraction time using microwave-assisted extraction. Work is on-going to expand the applicability of this new class of resins to other glycosides.